Kayan kwayar Halittar DNA / RNA na kwayar cuta (Shafi)
Ana amfani da Kit ɗin Tsabtace DNA don dacewa, hanzari kuma abin dogaro na yaduwar DNA kyauta (CFC-DNA) daga 1 mL zuwa 4 mL plasma / serum. Hanyar tsarkakewa ta dogara ne akan ƙirar chromatography na ginshiƙan centrifugal kuma yana amfani da resin mallakar Sigma don raba matrix. Za'a iya amfani da kit ɗin don keɓance CFC-DNA na masu girma dabam dabam daga samfuran plasma / jini mai sanyi ko kuma daskararre. Bugu da kari, za a iya daidaita girman karfin daga 25 μl zuwa 50 μl. Plasma / magani mai tsabta CFC-DNA zai kasance cikin walwala don daidaitawa tare da kowane aikace-aikacen da ke ƙasa, gami da PCR, ainihin lokacin ƙididdigar PCR, ƙwarewar PCR na methylation da binciken ƙwarewar Kudancin, microarray da NGS.
Rarraba DNA na masu girma dabam za'a iya kebe shi daga jini da samfurin jini
Za a iya ware kwayar halittar kwayar halitta ta DNA da kwayar cuta
Ya dace da kowane nau'in jini da magani fara samfurin girman (1 mL ~ 4 mL)
Za'a iya daidaita ƙarar ƙwanƙwasawa a cikin kewayon 50 μL ~ 100 μL don tattara tasirin DNA
Free kewaya DNA dauke da babu mai hanawa ana iya ware shi
Ana iya tsarkake DNA mai inganci a cikin mintina 40-45
Dace da Streck Cell-Free DNA BCT Tubes
Aikace-aikace
PCR
qPCR
Rubutun Kudancin
PCR mai mahimmanci Methylation
Tsarin CpG
Digesuntataccen narkewar enzyme
Gano cuta
Gano kwayoyin cuta
microarray
NGS
